Dye-buoyant-density-gradient-analysis of Rhizobium deoxyribonucleic acid
نویسندگان
چکیده
منابع مشابه
Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid.
Samples of bacterial deoxyribonucleic acid from bacteria having guanine plus cytosine (GC) contents in the range of 27 to 72 moles per cent GC were analyzed by optical melting (T(m)) and equilibrium buoyant density methods. The relation between these properties is shown to be linear. The relative value of 1.99 moles per cent GC per degree C change in T(m) is calculated, and a reference method f...
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Hybridization experiments were carried out between high molecular weight, denatured, agar-embedded deoxyribonucleic acid (DNA) and homologous, nonembedded, sheared, denatured (14)C-labeled DNA from a strain of Agrobacterium tumefaciens and Rhizobium leguminosarum (the reference strains) in the presence of sheared, nonembedded, nonlabeled DNA (competing DNA) from the same or different nomen-spec...
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The approach to equilibrium in a buoyant-density gradient is a transport process in a centrifugal field and may be used to measure a sedimentation coefficient. This process is interesting for several reasons. Densitygradient sedimentation equilibrium can be used for the determination of the molecular weight of high-molecular weight DNA samples homogeneous in buoyant density. Furthermore, buoyan...
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Covalently closed intracellular and viral simian virus 40 (SV40) deoxyribonucleic acid (DNA) were separately isolated from infected African green monkey cells (BSC-1) grown in culture. The two DNA species form overlapping bands centered at different positions in a propidium di-iodide-cesium chloride (PDI-CsCl) buoyant density gradient capable of separating closed DNA species with different supe...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1250103pa